SOUTHERN BLOTTING Presented by: Imran Fakhroni Nurkholis Nugroho Nino Radiansyah Indra Ardi Fauzi ... Detection Radioactive probes enable autoradiographic detection. Would you watch the animation? A. Yes B. No SUMMARY OF PROCEDURE 1. Extract and purify DNA from cells 2. DNA is restricted with ...
Date added: June 25, 2012 - Views: 155
Introduction to Northern/Southern Blotting Author: Michael Melzer Created Date: 6/6/2006 10:57:59 PM Document presentation format: On-screen Show Company: Plant Pathology Graduate Student Organization Other titles:
Date added: November 6, 2011 - Views: 211
Southern Blotting INVENTED BY BRITISH BIOCHEMIST EDWIN SOUTHERN * Purpose Used to determine whether a particular nucleotide sequence is present in different sources of DNA, and also to discover the size of the restriction fragment that contains the specific sequence Example Sickle Cell Can ...
Date added: November 28, 2011 - Views: 232
Southern Blotting A method to visualize specific segments of DNA– usually a ... probe for specific segment Wash away unbound probe Detect probes using x-ray film autoradiograph RFLP Animations Animation #1 Animation #2 Polymerase Chain Reaction PCR allows scientists to ...
Date added: April 15, 2012 - Views: 296
Size determined by gel electrophoresis and Southern blotting to produce pattern of ... Activities Review tutorial on Western Blot http://tinyurl.com/awaut3 Review VNTR animation http://tinyurl.com/dawjau Review DNA fingerprinting http://tinyurl.com/co2yml Review DNA forensics Problem set 1 ...
Date added: September 13, 2011 - Views: 161
Figure 20.9 Gel electrophoresis Figure 20.9 Gel electrophoresis Figure 20.11 Southern blotting of DNA fragments Figure ... allele Probe base-pairs with fragments Nitrocellulose blot 4 5 Radioactively labeled probe for -globin gene Southern blotting animation Northern blotting ...
Date added: September 4, 2011 - Views: 96
After gel electrophoresis Filter paper blots DNA off Radioactive probes added Autoradiography pinpoints sequence Southern Blot animation The scientific ... DNA fingerprinting Let’s take a brief look at some of these… What is gel electrophoresis? What is Southern blotting? The ...
Date added: May 22, 2013 - Views: 16
... heterozygous at coat color gene locus environmental effects on gene expression all of the above Gel Electrophoresis and Southern Blotting One ... Presentation PowerPoint Presentation PowerPoint Presentation PowerPoint Presentation PowerPoint Presentation Animation DNA ...
Date added: May 4, 2013 - Views: 11
Scene: Animation of labeled probe fragment pairing with target ... Animation of DNA/RNA probe preparations Animation of Complementary Base-Pairing Live shot of Researcher--Segue to Blotting Southern Blot Transfer Preparation Animation of Transfer Process Crosslink of DNA to Nitrocellulose ...
Date added: November 7, 2011 - Views: 28
This would take about 3 hours to perform in lab. PCR Animation DNA Fingerprinting Used to identify individuals by their repeat regions (usually STR) regions: ... (Southern blotting) A radioactive primer is designed that will be complementary to unique regions (STR, etc, regions).
Date added: February 13, 2013 - Views: 32
... the role of a gene in an organism Several techniques are used to analyze the DNA of genes Gel Electrophoresis and Southern Blotting One indirect method of rapidly analyzing and comparing genomes is gel electrophoresis This technique uses a gel as a ... Base pairing occurs. 2 Animation: ...
Date added: May 13, 2013 - Views: 8
Web animation of a cDNA microarray experiment Yeast genome on a chip Brief outline of steps for producing a microarray cDNA probes attached or synthesized to solid support Hybridize targets Scan ... 3’ 3’ – CTTAAG – 5’ (cut) The Southern blotting technique * Title ...
Date added: October 15, 2011 - Views: 91
Scientific Applications Understanding DNA Sequencing organisms' genomes DNA fingerprinting for identification Southern Blotting Southern Blotting Southern Blotting Forensic Microbiology PCR Primer for a specific ... Recombinant DNA Technology Table 9.1 Figure 9.2 Figure 9.3 ANIMATION PCR: ...
Date added: October 26, 2011 - Views: 69
Several techniques are used to analyze the DNA of genes: Gel Electrophoresis, restriction fragment analysis, Southern blotting, DNA sequencing. ... For the Discovery Video Transgenics, go to Animation and Video Files.
Date added: February 9, 2012 - Views: 33
* * Figure 20.11 Research Method: Southern Blotting of DNA Fragments * * Figure 20.12 Research Method: ... The fragments are mixed, and DNA ligase is added to bond the fragment sticky ends © 2011 Pearson Education, Inc. Animation: ...
Date added: March 16, 2013 - Views: 10
Southern blotting c. Northern blotting Restriction Mapping Animation: Restriction Mapping 1. Cloned DNA can be cut with restriction enzymes and electrophoresed on agarose gels and visualized with ethidium bromide, in order to map its restriction sites (Figure 8.13). 2.
Date added: June 5, 2012 - Views: 22
... Animation http://188.8.131.52/pub/flash/4/4.html `http://www.dnalc.org/ddnalc/resources/electrophoresis.html Southern blotting Southern blotting was ... Automated DNA Sequencer Sequence chromatogram Animation of DNA sequencing http://www.dnalc.org/resources/animations/cycseq.html ...
Date added: March 27, 2014 - Views: 1
Hybridization DNA hybridization Southern blotting (pg75) Southern Blot ... Primer extension Fig. 3.8 The Polymerase Chain Reaction Go to Animation courtesy of the following websites: References http://faculty.plattsburgh.edu/donald.slish/PCRmov.html ...
Date added: July 1, 2012 - Views: 16
Examples of Applications: Southern blotting, DNA chips, and FISH Nucleic Acid Hybridization: DNA Chip Fig 10.17 Fluorescent In Situ Hybridization (FISH) Fig 10 ... Practice ANIMATION Dichotomous Keys: ...
Date added: December 5, 2011 - Views: 70
Contents RFLP technology Isolating DNA Restriction digestion and gel electrophoresis DNA transfer by Southern blotting DNA hybridisation Interpreting RFLP bands Advantages and ... Summary Animation http://bcs.whfreeman.com/thelifewire/content/chp16/1602001.html http://www.biostudio ...
Date added: May 4, 2013 - Views: 16
Southern, Northern & Western blotting DNA sequencing Polymerase chain reaction (PCR) DNA microarrays RNA interference (RNAi) ... DNA Sequencing Dideoxy DNA Sequencing Animation- MCB Chapter 9 Characterization: ...
Date added: May 4, 2013 - Views: 5
Examples of Applications: Southern blotting, DNA chips, and FISH Figs. 10.17 and 10.18 PLAY Animation: Dichotomous Keys (The Microbiology Place) Method used to establish cladogram for microorganisms? ...
Date added: June 12, 2012 - Views: 61
... Dichotomous Key Building a Cladogram Building a Cladogram Building a Cladogram What is tested in Western blotting and Southern blotting? ... Practice ANIMATION Dichotomous Keys: Sample with Flowchart ANIMATION Dichotomous Keys: ...
Date added: May 3, 2013 - Views: 33
Biology Animation Library/DNA Restriction Restriction enzymes are used to make recombinant DNA Restriction enzymes cut both strands, ... Southern Blotting: uses radioactive labels to identify the fragments of interest uses Non-coding DNA uses restriction fragment length polymorphisms ...
Date added: April 23, 2014 - Views: 3
Chapter 20 Biotechnology * Figure 20.9 Gel electrophoresis * Figure 20.9 Gel electrophoresis * Figure 20.9 Gel electrophoresis * * Figure 20.10 Using restriction fragment analysis to distinguish the normal and sickle-cell alleles of the β-globin gene * * Figure 20.11 Southern blotting of DNA ...
Date added: May 3, 2014 - Views: 5
What is Southern blotting? 9-17 Why do RFLPs result in a DNA fingerprint? 9-18 Of what value is the plant ... Clone DNA for recombination Amplify DNA to detectable levels Sequence DNA Diagnose genetic disease Detect pathogens ANIMATION PCR: Components ANIMATION PCR: Overview Incubate ...
Date added: May 2, 2013 - Views: 25
... Retrotransposon movement Animation Quiz 5 - Transposons: ... If the probe binds to the membrane, then the probe sequence is present in the sample. Southern Blotting DNA Sequencing Determination of nucleotide sequences (Sanger method, ...
Date added: February 5, 2014 - Views: 5
... method for confirming the presence of a DNA segment within an entire genome than other conventional methods like Southern blotting FISH Procedure Denature the chromosomes Denature the probe Hybridization Fluorescence staining Examine slides or store in the dark FISH Procedure FISH Uses ...
Date added: February 17, 2013 - Views: 184
... Protein Synthesis Animation Biostudio Protein Synthesis Animation Polymerase Chain ... differences in these sequences can be used to distinguish individuals sequences are separated by gel electrophoresis Southern blotting transfers separated fragments to nylon membrane Hybridization ...
Date added: November 29, 2013 - Views: 1
FISH, Southern transfer (blotting) and DNA microarray analyses involve hybridization. Hybridization. Hybridization(1) Fluorescent in situ Hybridization. FISH. Use DNA or RNA probes for hybridization. ... Animation. 1-Endo-2-Exo-Cleave . Middle of Chain.
Date added: February 5, 2012 - Views: 46
Southern blotting: Transfer DNA from ... DNA polymerase cannot add the next complementary base synthesis stops Sanger Method Animation http://www.mefeedia.com/watch/21777157 Human Genome Project To determine the genetic sequence of the 46 human chromosomes Used similar sequencing technique, ...
Date added: December 28, 2013 - Views: 2
Southern blotting: Transfer DNA from ... Southern Blot Animation http://www.sanger.ac.uk/Info/Intro/gfx/fred_bw.jpg Chain termination resulting in different DNA fragment lengths Separate different DNA lengths by gel electrophoresis, ...
Date added: May 20, 2014 - Views: 1
... Or DNA can be removed from the gel by Southern Blotting References www.biotech.iastate.edu/publication/ppt-presentations Kreuzer, H., Massey, A., ... The gel is then visualized - UV light causes the bands of DNA to fluoresce Animation More……
Date added: July 6, 2013 - Views: 4
Revolution in cloning: Polymerase Chain Reaction (PCR)--1986 PCR Animation Please click here. 1st cycle ... Southern blotting (DNA) 2. Northern blotting (RNA) 3. Western blotting (protein ...
Date added: June 3, 2013 - Views: 11
Animation file made by Justin H. Lo may help you understand how replication is related with homologous recombination: ... (Southern blotting). (A) ...
Date added: June 2, 2013 - Views: 5
... of DNA molecules Such analysis can rapidly provide comparative information about DNA sequences Gel Electrophoresis and Southern Blotting One indirect method of rapidly analyzing and comparing genomes is gel electrophoresis This ... Restriction Enzymes Animation: Cloning a Gene ...
Date added: May 13, 2013 - Views: 3
Southern blotting, DNA chips, and. FISH . Nucleic Acid Hybridization: DNA Chip. Fig 10.17. Fluorescent In Situ ... Add DNA or RNA probe attached to fluorescent dye. for S. aureus. Dichotomous Key. ANIMATION Dichotomous Keys: Practice. ANIMATION Dichotomous Keys: Sample with Flowchart. ANIMATION ...
Date added: December 26, 2012 - Views: 31
See Chapter 9 animation http://bcs.whfreeman.com/lodish7e/#800911__811944__ Fig. 9.3 ... saliva, bone or any other tissue (often found at a crime scene) Can be done by southern blotting with an appropriate probe or by a PCR method using appropriate primers Can use single locus probes ...
Date added: January 21, 2013 - Views: 30
Figure 11.2 Agarose gel electrophoresis of DNA. Figure 11.4 Southern blotting. Figure 11.5 Major steps in gene cloning. ... gene libraries made by cloning random genome fragments © 2012 Pearson Education, Inc. Animation: ...
Date added: October 6, 2012 - Views: 55
* Overview DNA Cloning Restriction Enzymes Gel Electrophoresis and Southern Blotting Gene Expression Detection Applications of Gene Technology Medical Environmental Agricultural Ethical ... Base pairing occurs. 2 Fig. 20-UN5 Fig. 20-UN6 Animation Please note that due to differing operating ...
Date added: May 2, 2013 - Views: 20
... the role of a gene in an organism Several techniques are used to analyze the DNA of genes Gel Electrophoresis and Southern Blotting Gel electrophoresis uses a gel as a molecular sieve to separate nucleic acids by size A current is applied to the ... go to Animation and Video Files ...
Date added: March 14, 2012 - Views: 32
... gels for DNA PAGE for proteins Single digest uses 1 enzyme Double digest produces 2 restriction patterns Interactive Animation, Simplified Blotting Goes from the gel to filter paper to make and autoradiograph Southern blot: uses RNA to probe DNA strands (SB2) ...
Date added: March 4, 2012 - Views: 39
... Acid Hybridization A similar process can be used to screen isolated fragments of DNA for the sequences of interest Southern blotting ... you can collect your normal and cancer cells and extract the gene expression products – mRNA Animation Denaturation (94°C) Annealing (30-70°C ...
Date added: August 5, 2013 - Views: 6
For example, Western blotting ... bind RNA polymerase & initiate transcription http://www-class.unl.edu/biochem/gp2/m_biology/animation/gene/gene_a2 ... Signal Transduction Pathways TGF-β Gene Therapy Immunotherapy Herceptin Chemotherapy Gleevac Gene Therapy Southern Blotting ...
Date added: August 3, 2013 - Views: 13
This is done by ‘Southern Blotting’. Filter ... gel electrophoresis DNA fragments loaded onto a gel Current is applied DNA molecules carry a residual –ve charge See animation Activity 2.20 Fragments of DNA molecules attracted and move towards + ve electrode Small fragments will ...
Date added: December 26, 2013 - Views: 6
Other Applications of DNA Fingerprinting Parentage test Endangered species or Chinese herbs identification Animation 1: Southern Blotting http://www.dnalc.org/resources/BiologyAnimationLibrary.htm Animation 2: ...
Date added: March 16, 2012 - Views: 19
to a probe Southerng Blotting fig* Sumanas, Inc. animation - DNA electrophoresis & blotting * one can detect ... (recognition sequence: 5'-C^TNAG-3') Southern blotting probe: fragment of ß-globin coding ...
Date added: October 15, 2011 - Views: 36
... Talking Glossary of Genetic Terms.htm Talking Glossary of Genetic Terms DNALC Biology Animation Library.htm Animation Library Thank ... villi and amnionic fluid Mouth wash Introduction DNA isolation RNA isolation Introduction Molecular Bio-techniques Blotting Southern ...
Date added: May 23, 2013 - Views: 16
ELISA One of many variants of the technique http://www.immunospot.com/elisa-animation.html Advantages of ELISA Sensitive: ... The choice of material to test DNA most common; tested by PCR Sometimes tested by Southern blotting RNA RT-PCR allow to test genes directly, ...
Date added: June 18, 2012 - Views: 47
DNA is transferred by Southern blotting to a membrane. Membrane ... Molecular Diagnostics Characteristics of a Detection System Immunological Diagnostic Procedures ELISA ELISA ELISA Animation ELISA Lab/Detection of HIV Detection of HIV DNA Diagnostic Systems DNA Hybridization DNA ...
Date added: November 18, 2013 - Views: 6