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Combined Cosmids (ppt) - University of Illinois at Chicago - UIC

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Combined Cosmids (ppt) - University of Illinois at Chicago - UIC

Alcaligenes faecalis combined cosmids map with 70 ORFs Amino acid permease L-Kynurenine hydrolase Transcriptional regulators orf77 gntR orf416 orf153

http://www.uic.edu/depts/mcmi/faculty/silver/Combined%20Cosmids%20Map.ppt

Date added: October 24, 2011 - Views: 47

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PowerPoint Presentation

cosmids Cosmids are plasmid vectors that contain one or two “cos sites”. (The cos site and the length between 2 cos sites is the only requirement for DNA to be

http://genemol.org/molecular-bio/7-Artificial-chromosomes/YAC-BAC-PAC.ppt

Date added: October 7, 2011 - Views: 198

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Vectors - College of Computer, Mathematical, and Natural Sciences

Cosmids combine essential elements of a plasmid and Lambda systems. Cosmids are extracted from bacteria and mixed with restriction endonucleases. Cleaved cosmids are mixed with foreign DNA that has been cleaved with the same endonuclease.

http://www.life.umd.edu/grad/MLfsc/res/RecombomamtDNA&Medicine.ppt

Date added: July 18, 2013 - Views: 27

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Introduction to Vectors - Rutgers University

Cosmids - Plasmids with phage packaging sites p. 2-16 4. YACs - Yeast Artificial Chromosomes p. 2-17 Vector Insert Size Vector Type Cloned DNA (Kb) Plasmid 20 Phage ...

http://beadle.rutgers.edu/MBB/315/Lec/315-Ch2.ppt

Date added: November 12, 2011 - Views: 92

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PowerPoint Presentation

Cosmids, however, always form colonies and not plaques. Also the clone density is much lower with around 105 - 106 CFU per µg of ligated DNA.

http://forest.mtu.edu/classes/fw3300/BL3300%202011/2011%20%20Chapt%2003%20091904%20cpj.ppt

Date added: May 21, 2012 - Views: 38

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justc/courses/RecombDNA/Week6. ppt

Cosmids BACs PACs YACs Cosmids replicate as high copy number plasmids--tend to be unstable, deleting insert DNA (to reduce drag on cells) ...

http://web.pdx.edu/~justc/courses/RecombDNA/Week6.ppt

Date added: October 7, 2011 - Views: 61

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PowerPoint Presentation

Vectors for large DNA inserts: Examples: Cosmids PACs BACs Plasmid Vectors Origin of Replication b-lactamase: breaks down ampicillin ...

http://faculty.smu.edu/borr/ClassNotes/10-14-05.ppt

Date added: May 13, 2013 - Views: 15

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PowerPoint Presentation

... Life cycle of a bacteriophage 2-8 Constructing a genomic library in phage 2-9 Constructing a genomic library in cosmids 2-10 Constructing a genomic library in YACs * Look at vector first, Where are all the the sites that you know about? * * *

http://wssp.rutgers.edu/StudentScholars/WSSP06/WSSP-06_files/Figures/WSSP06-Ch2-LIbraries.ppt

Date added: May 5, 2013 - Views: 21

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PowerPoint Presentation

... & Cosmids (plasmid + l cos site & can be pckged into phage capsid) Transgenic Plant: Vector = Agrobacterium tumefaciens plasmid called Ti (causes crown gall tumors). Plasmid codes for virulence gene and regulates pant growth ...

http://www.oucom.ohiou.edu/dbms-james/Winter_2003/Recomb%20DNA%20Tech.ppt

Date added: August 2, 2013 - Views: 12

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PowerPoint Presentation

Other vectors include bacteriophage, cosmids, etc. pUC18 Plasmid Origin of replication DNA fragment up to 5 kbp can insert IV. Cloning with a Plasmid Vector Recombinant DNA Transformation Selection for cells carrying recombinant plasmids by plating cells on media with antibiotic.

http://people.cst.cmich.edu/schis1j/bio326-02/lectures/11-8.ppt

Date added: January 13, 2013 - Views: 7

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Chapter 12

Plasmid Placement in Cell PowerPoint Presentation Phage vectors Phage Insertion Cosmids YACs YACs YACs Essential elements for YACs PowerPoint Presentation Particle Gun We can insert the gene into cells – Now what? Basic Steps ...

http://www3.nd.edu/~ljordan/butterfly/genetics/biotech.ppt

Date added: May 3, 2013 - Views: 16

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PowerPoint Presentation

... screening labelling PowerPoint Presentation PowerPoint Presentation phage and cosmid screening phages cosmids PACs BACs PowerPoint Presentation YACs PowerPoint Presentation PowerPoint Presentation Shotgun sequencing Chromosome Walking Chromosome jumping. Southern (DNA) and ...

http://genemol.org/molecular-bio/technics.ppt

Date added: October 12, 2011 - Views: 14

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PowerPoint Presentation

Bacteriophage-based Cosmids: Linear DNA molecules used for genomic and cDNA cloning. Bacterial host. Insert size range < 20kb. Bacterial Artificial Chromosomes (BACs): Circular DNA molecules used for cloning very long segments of genomic DNA. Bacterial ...

http://users.umassmed.edu/maria.zapp/Block2Lecture1%2011_01_04.ppt

Date added: October 7, 2011 - Views: 97

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PowerPoint Presentation

Mobilizing DNA: vectors for propagation in E. coli Plasmids Bacteriophage M13 Lambda Cosmids and BACs * 5´ ... G^A A T T C ... 3´ 3´ ... C T T A A^G ... 5´ 5´ ... G^ 3’ 5’ A A T T C ... 3´ 3´ ... C T T A A 5 ...

http://web.pdx.edu/~justc/courses/RecombDNA/Week4.ppt

Date added: October 3, 2011 - Views: 82

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Plasmids - e-campus21

... pBK Cosmids pWE15/16, Supercos Shuttle plasmids Recombinant selection Recombinant selection is the ability to determine which clones contain an inserted piece of DNA Three main types Antibiotic resistance Lac Z’ (a complementation) ...

http://www.e-campus21.com/courseware/teachaids/bmg/mg1/mg1/plasmids.ppt

Date added: May 3, 2013 - Views: 41

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PowerPoint Presentation - DNA Libraries - Biology Department ...

... Transformation (via bacterial mechanisms); plasmids BACS YACS Transduction (via virus mechanisms); phage cosmids Cloning Step 3 ...vectors contain selectable markers, only cells that contain vector DNA will survive selection, ...

http://www.biol.wwu.edu/young/321/lectures/lg8.ppt

Date added: October 6, 2011 - Views: 124

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PowerPoint Presentation

Larger DNA fragments can be cloned in cosmids and BACs Vector Preparation In order to use a vector for cloning, sequencing, etc., it is necessary to isolate the vector in a highly purified form. Routinely done by most labs.

http://wssp.rutgers.edu/StudentScholars/WSSP05/WSSP-05_files/Figures/WSSP05-7-5-Vectors.ppt

Date added: May 20, 2013 - Views: 29

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DNA cloning intro.ppt - Biology

Clone large inserts of DNA: size ~ 45 kb Features: Cosmids are Plasmids with one or two Lambda Cos sites. Presence of the Cos site permits in vitro packaging of cosmid DNA into Lambda particles COSMID VECTOR Thus, ...

http://biology.hunter.cuny.edu/molecularbio/Class%20Materials%20Fall%202012%20710.LC/ANiMATED%20PPT%20to%20help%EF%80%A8/DNA%20cloning%20intro.ppt

Date added: January 23, 2013 - Views: 28

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Lecture 16: Genomics - University of Alabama at Birmingham

... by PCR to determine inheritence patterns Produce linkage map with nearby loci Physical Maps Radiation Hybrid/YACs/Cosmids Restriction Sites Sequence Tagged Sites 100 Kb resolution needed 30,000 STS’s Expressed Sequence Tags Detection PCR Hybridization FISH Fluoresecent in situ ...

http://www.genome.uab.edu/MIC753/files/16_Genome.ppt

Date added: November 6, 2011 - Views: 78

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Plasmids and Vectors - Molecular Biology for Secondary ...

Plasmids and Vectors Instructor Supplement to pGlo Bacterial Transformation A more detailed look at plasmids Cloning into a Plasmid Asilomar Conference Screening bacteria by replica plating Cosmids Other Vectors BACs (Bacterial artificial chromosomes) Large low copy number plasmids (have ori and ...

http://unlmbsc.files.wordpress.com/2007/08/plasmids-and-vectors.ppt

Date added: October 16, 2011 - Views: 178

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PowerPoint Presentation

... allow reproduction of gene plasmids phages cosmids part viral, part plasmid BACs - bacterial artificial chromosomes YACs - yeast artificial chromosomes Cloning Cloning Use SAME restriction endonuclease to cut out target gene and open vector Must use an enzyme that produces sticky ends!

http://people.uncw.edu/doddd/Biology%20335/PowerPoints/DNAtech.ppt

Date added: March 10, 2012 - Views: 12

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Chapter 3

... Types of vectors Bacterial plasmid bacteriophage cosmids bacterial artificial chromosome yeast artificial chromosome 6-12 25 35 300 200-1000 Maximum insert size (kilobases or kb [1000bp]) Practical Features of DNA Cloning Vectors (Plasmids) origin of replication ...

http://www.montana.edu/wwwmb/coursehome/BIOM107CS/Lectures/Chapter%203.ppt

Date added: October 10, 2012 - Views: 37

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PowerPoint Presentation

... lambda (~ 15-20 kb) cosmids for larger DNA molecules (~ 40-50 kb) BAC: bacterial artificial chromosome (~ 100-300 kb) YAC: yeast artificial chromosome (~ 1000 kb) The vector is cut with the restriction endonuclease and mixed with the DNA restriction fragments to be cloned.

http://site.iugaza.edu.ps/fsharif/files/2010/02/MB-62.ppt

Date added: May 3, 2013 - Views: 11

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Molecular Cloning - University of Hawaii

Plasmids – used in cloning small segments of DNA (10-15 kb) Cosmids – plasmids containing DNA sequences (cos) from bacteriophage λ used to clone larger fragments of up to 45 Kb Bacteriophage λ ...

http://abe.leeward.hawaii.edu/Summer07/Introduction%20to%20Molecular%20Cloning.ppt

Date added: November 5, 2011 - Views: 74

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PowerPoint Presentation

... from GeneScan and Ensembl are occasionally contradictory chicken also contained small mini-chromosomes that resemble cosmids which were lumped into the category of ChrUn, ...

http://instructional1.calstatela.edu/jmomand2/2004/presentations/ppt/ng.ppt

Date added: May 6, 2013 - Views: 3

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Part II: Recombinant DNA Technology.ppt - Rajesh Banu

Title: Part II: Recombinant DNA Technology.ppt Author: ITR Last modified by: Konkuk university Created Date: 1/20/2002 6:56:56 PM Document presentation format

http://rajeshbanu.com/ebt/molecular%20breeding_RDT_2.ppt

Date added: May 2, 2013 - Views: 17

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PowerPoint Presentation

1 Plasmid vectors 2 Bacteriophage vectors 3 Cosmids 4 BACs & YACs Plasmid vectors Advantages: Small, easy to handle Straightforward selection strategies Useful for cloning small DNA fragments (< 10kbp) Disadvantages: Less useful for cloning large DNA fragments ...

http://faculty.ksu.edu.sa/73614/amustafa/355/chapter3.ppt

Date added: December 10, 2013 - Views: 4

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PowerPoint Presentation

The physical map of the C. elegans genome was put together after ordering then sequencing genomic YACs and cosmids. * The genetic and physical maps can be aligned with each other. The spacing can vary between genetic markers and their physical genes; however, ...

http://beadle.rutgers.edu/MMG/502files/502-Ce/502-Ce-L2.ppt

Date added: June 27, 2012 - Views: 11

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Cloning into Plasmids - Buffalo State College

... 8-20 kb Cosmids - an extrachromosomal circular DNA molecule that combines features of plasmids and phage; cloning limit - 35-50 kb Bacterial Artificial Chromosomes (BAC) - based on bacterial mini-F plasmids. cloning limit: ...

http://faculty.buffalostate.edu/eastondp/dpeastonbiolog/Bio%20450/Cloning%20into%20Plasmids%20-Riboprobes.ppt

Date added: December 2, 2011 - Views: 80

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PowerPoint Presentation

... DNA splicing * * * * * * * Table 3.1 * * * Table 3.1 * * Figure 3.3b * * * Figure 3.3b * * Figure 3.5 * * * Figure 3.5 Cosmids Contain cos ends of lambda DNA, a plasmid origin of replication, and genes for antibiotic resistance, ...

http://ap-biology-lab.wikispaces.com/file/view/chapter+3.ppt

Date added: June 30, 2014 - Views: 1

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Lambda Vectors and their replication - Department Of ...

Cosmids- are hybrid vectors of l phage and plasmids. They can replicate their DNA in the cell with a plasmid and be packaged like a phage. They can insert up to 50kb. 4. Yeast artificial chromosomes (YAC)- primarily used in genome sequencing projects.

http://biology.hunter.cuny.edu/molecularbio2/Seminar%20Presentations/February%2027/Lambda%20Vectors%20and%20their%20replication.ppt

Date added: September 30, 2013 - Views: 22

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Gene cloning

Cosmids are extracted from bacteria and mixed with restriction endonucleases. Cleaved cosmids are mixed with foreign DNA that has been cleaved with the same endonuclease.

http://www.kau.edu.sa/Files/0002512/Subjects/Gene%20cloning3.ppt

Date added: March 19, 2014 - Views: 1

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Section D - Prokaryotic and Eukaryotic Chromosome Structure

H3 Cosmids, YACs and BACs — Cloning large DNA fragments Analysis of eukaryotic genes and the genome organization of eukaryotes requires vectors with a larger capacity for cloned DNA than plasmids or phage Human genome (3 x 109 bp ...

http://fzswx.gxu.edu.cn/course/Section%20H%20-%20Cloning%20vectors.ppt

Date added: February 13, 2012 - Views: 10

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Part II: Recombinant DNA Technology.ppt - Cabrini College

Title: Part II: Recombinant DNA Technology.ppt Author: ITR Last modified by: Sherry Fuller-Espie Created Date: 1/20/2002 6:56:56 PM Document presentation format

http://www.cabrini.edu/sepchedna/2003%20Workshop/Power%20Point%20Presentations%202003/Part%20II%20Recombinant%20DNA%20Technology_NO_FIGS.ppt

Date added: October 7, 2011 - Views: 234

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Foundations in Microbiology - South Georgia College

The gene is inserted into a vector (plasmid, virus, cosmids) that will insert the DNA into a cloning host. Cloning host is usually bacterium or yeast that can replicate the gene and translate it into a protein product.

http://faculty.sgc.edu/sundram/2215/Chapter10_lecture.ppt

Date added: December 13, 2011 - Views: 64

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Genome Sequencing - Kennesaw State University College of ...

... a set of cDNA sequences to a set of cosmids, contig sequences found by two alternative assembly procedures (for example, phrap and xbap) to each other, or phrap contigs to the final edited cosmid sequence. It is slower but more sensitive than BLAST.

http://science.kennesaw.edu/~jsalern3/Site/bioinfo/Genome%20Sequencing.ppt

Date added: September 12, 2011 - Views: 40

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Biotechnology and Genetic Engineering-PBIO 450/550

Title: Biotechnology and Genetic Engineering-PBIO 450/550 Author: showalte Last modified by: Allan Showalter Created Date: 9/8/2003 3:48:03 PM Document presentation format

http://www.ohio.edu/plantbio/staff/showalte/MCB%20720/MCB7200%20Lecture%203.ppt

Date added: February 13, 2013 - Views: 24

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Gene Cloning

... 8-20 kb Cosmids - is a combination of plasmids and phage; cloning limit - 35-50 kb Bacterial Artificial Chromosomes (BAC) - based on bacterial mini-F plasmids. cloning limit: 150-300 kb (350kb in some books).

http://xa.yimg.com/kq/groups/24240295/1734630456/name/lecture+04.ppt

Date added: February 18, 2014 - Views: 3

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Fragile X Syndrome - University of Wisconsin–Eau Claire

... Created a cosmid library from the YAC clone Cosmid subclones used to screen a cDNA library of human fetal brain RNA The cosmids hybridized to a portion of a gene designated as FMR1 Pedigree of a family with the Fragile X mutation segregating FMR1 Expression Northern Blot of FMR1 was done ...

http://www.uwec.edu/anderju/biol409/presentations/Fragile%20X%20Syndrome.ppt

Date added: June 20, 2012 - Views: 38

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Biotechnology and Genetic Engineering-PBIO 450/550

... to make and screen cDNA libraries Several different cloning vectors including plasmids, bacteriophage lambda (l), and cosmids ...

http://www.ohio.edu/plantbio/staff/showalte/PBIO%20450%20&%20550/PBIO450Lecture308.ppt

Date added: October 6, 2011 - Views: 16

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Chem 360 Lecture slides - Weston

Cosmids- artificially generated ... Radiolabeled vs. fluorescent tag Isolation of Gene of Interest Isolation of Gene of Interest Use of Antibodies Ab ppt protein Protein being synthesized on mRNA Generate cDNA from mRNA reverse transcriptase DNA polymerase Must have protein in ~pure ...

http://www.weston.org/schools/ms/biologyweb/heredity/handouts/recombinant_dna_tech_ppt.ppt

Date added: May 2, 2013 - Views: 22

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Recombinant DNA Technology - NIU Department of Biological ...

Use these phage to infect new E. coli. Cosmids are similar to phage vectors: use lambda, but remove all but the ends (cos sites), ori, and selectable marker. Package in vitro--becomes a large (50 kb) ...

http://www.bios.niu.edu/johns/humgen/Recombinant_DNA_Technology.ppt

Date added: October 7, 2011 - Views: 239

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Chapter 3

... Plating cells on agar surface to promote colony formation Cloning Types of vectors Bacterial plasmid bacteriophage cosmids bacterial artificial chromosome yeast artificial chromosome Practical Features of DNA Cloning Vectors (Plasmids) origin of replication ...

http://www.montana.edu/wwwmb/coursehome/mb105/Lectures/Chapter%203.ppt

Date added: September 27, 2011 - Views: 80

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PowerPoint Presentation

AD000092 /FEATURE=cds#6 /DEFINITION=CH19HHR23 Homo sapiens DNA from chromosome 19p13.2 cosmids R31240, R30272 and R28549 containing the EKLF, GCDH, CRTC, and RAD23A genes, genomic sequence. 1753_s_at.

http://combio.cs.brandeis.edu/hong/CS230a/CourseInfo/DetectDEG.ppt

Date added: June 22, 2012 - Views: 30

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classes.biology.ucsd.edu/old.web.classes/bimm120.WI06/john ...

... Cosmids can be packaged into lambda capsids and then manipulated as a phage Can also exist in the cell like a plasmid Can be used to clone very large pieces of DNA Artificial chromosomes - can be yeast or bacterial; ...

http://classes.biology.ucsd.edu/old.web.classes/bimm120.WI06/john_buchanan_lec2.ppt

Date added: September 15, 2012 - Views: 18

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Recombinant DNA Technology for the non-science major.

Cosmids (artificial constructions) A prokaryotic vector should: 1. Be capable of autonomous replication independent of the main bacterial chromosome 2. Be easy to isolate, i.e. small. 3. Be non-toxic to host cells. 4. Have space ...

http://cogs.csustan.edu/~tom/bioinfo/ppt/Day3.ppt

Date added: October 16, 2012 - Views: 19

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Slide 1

... Cosmids can be packaged into lambda capsids and then manipulated as a phage Can also exist in the cell like a plasmid Can be used to clone very large pieces of DNA Artificial chromosomes - can be yeast or bacterial; ...

http://classes.biology.ucsd.edu/bimm120.WI07/genetics2_doran_fa06.ppt

Date added: September 7, 2012 - Views: 18

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Recombinant DNA Technology - NIU Department of Biological ...

I hope Cloning Vector Types For different sizes of DNA: plasmids: up to 5 kb phage lambda (λ) vectors (also cosmids): up to 50 kb BAC (bacterial artificial chromosome): 300 kb YAC (yeast artificial chromosome): 2000 kb Expression vectors: ...

http://www.bios.niu.edu/johns/bioinf_intro/Recombinant%20DNA%20Technology.ppt

Date added: May 8, 2012 - Views: 46

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Incorrect Baseline settings can have an adverse affect on ...

... Default Set-up and BiSulSeq Profile “Vish Profile” Cosmids: Sequence Context Constraints: Run of G’s: Default Set-up and Profile (AutoSeq1) Run of G’s: dGTP Chemistry, AutoSeq1 profile GC Rich Template: Generic Set up, AutoSeq1 Profile GC Rich Template: ...

http://www.uvm.edu/medicine/vtcancercenter/documents/DNASem5.11.07.ppt

Date added: July 26, 2013 - Views: 13

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Inquiry into Life Twelfth Edition - McGraw-Hill

... labeled nucleic acid with sequence matching the gene of interest Cosmids Cosmids are designed for cloning large DNA fragments Behave as plasmid and phage Contain cos sites, ...

http://highered.mcgraw-hill.com/sites/dl/free/0072995246/444682/chapt04_lecture.ppt

Date added: October 13, 2011 - Views: 75